FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt60120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt60120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15290299
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19950.021.09748544
GTGTGCG27800.020.6012738
TATGCCG20350.020.34372746
CGGGCGC29700.019.97955115
GGCGGGC30100.019.82878913
CGCTATG44650.018.69859729
CGGCAAT49550.017.7559091
TAGCCCG36050.016.93876534
GCGGGCG34950.016.8796214
GGCTGCC50650.016.55162243
CCCTGTG50650.016.55162263
GCCAGTA35450.016.4481431
GTGCGAG34800.016.35787810
GTATGCC26500.016.27338445
GTCGCTA51050.016.21929727
TCGGGCG40400.015.7986927
AACGTCG54000.015.65273824
GCACACG37450.015.56871811
CCGACAT59550.015.2377341
TGTGCGA39550.015.1783669
Produced by FastQC (version 0.11.2)