FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt60120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt60120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13564176
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20600.019.0920444
TATGCCG21300.018.30263146
GTGTGCG15600.017.6929768
CGGGCGC14500.017.3687915
GGCGGGC16600.015.37947613
GTATGCC26250.015.11413345
GCACACG33650.014.55870511
TCGTATG28250.014.2883543
ATGCCGT28550.014.13820947
CGGGCAT23350.013.5937121
GCCAGTA30200.013.4806911
ACGTCTG38050.013.3283815
GCGGGCG19050.013.22033814
CGCGTAT10700.013.2201751
CGGCAAT37150.013.1876261
CGCGCGT18500.012.68090465
ATCGGGC24750.012.5462996
CGCTATG27900.012.36546729
TCGGGCG22750.012.2840257
CACACGT40250.012.25710912
Produced by FastQC (version 0.11.2)