FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt1120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt1120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18285481
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29000.022.12729544
TATGCCG28900.021.72635846
GTGTGCG26750.020.1202968
CGGGCGC21900.020.00664115
CGCGTAT16800.018.2778971
GGCGGGC24250.018.06795313
GTATGCC36600.017.24977345
ATGCCGT37550.016.90523747
TCGTATG38550.016.55620243
GCCAGCG50550.016.447934
TAGCCCG29550.016.11150734
GCCAGTA43000.016.0474851
CGCTATG42100.015.81574929
GTCCTAT53000.015.29696943
CCTATAA55200.015.06230345
GTGCGAG35800.015.03365110
GCGGGCG29350.014.92829514
ACTGACC43250.014.9166532
AGGTGCC55950.014.860435530
ACCCGTA37200.014.56037232
Produced by FastQC (version 0.11.2)