FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt1120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt1120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15793497
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA41700.023.7464471
TATGCCG22200.021.60104446
CGTATGC22700.021.58119444
GTGTGCG29200.020.9131688
CGCTATG47800.019.55940829
CGCGTAT18750.019.1374861
CGGGCGC33500.019.0520315
GGCGGGC34600.018.94493913
GTCGCTA50450.018.8056627
GTCCTAT47900.017.28576943
CGGCAAT51900.017.21811
GGCTGCC55750.017.20334443
GTGCGAG35750.017.17786810
AACGTCG56950.016.8410124
GCCAGCG49950.016.783634
CCGACAT61000.016.4594751
CCCTGTG57150.016.41971463
TAGCCCG39850.016.3624334
CCTATAA50950.016.25099645
ATGCCGT31300.016.20264447
Produced by FastQC (version 0.11.2)