FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt10120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt10120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15199206
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG23400.020.7894428
TATGCCG21750.019.35142746
CGTATGC22400.019.25193844
CGCGTAT15350.017.3072321
CGCTATG40250.016.97116729
CGGGCGC26700.016.66833915
GTCGCTA41550.016.44028927
GCCAGTA35900.016.145821
GGCGGGC28300.016.09183913
GTATGCC27000.015.97197745
GTGCGAG31750.015.4300910
GTCACGC28350.015.33309729
CGGCAAT46100.015.192891
TCGTATG30650.014.85784743
GGCTGCC47400.014.33838843
GCGGGCG31050.014.3331614
CACGCCA32050.014.31647231
ATGCCGT29950.014.16839747
CCGACAT51950.014.1461851
TAGCCCG31500.014.12835434
Produced by FastQC (version 0.11.2)