FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt10120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt10120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16632240
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG26600.021.141918
CGGGCGC28450.020.49364515
GGCGGGC30250.019.61633313
CGTATGC23050.019.15798844
CGCGTAT16400.018.9341031
CGGCAAT49100.017.6375521
GCGGGCG33600.017.35250714
TATGCCG25550.017.28342846
CGCTATG41200.016.99851429
TAGCCCG36450.016.27952634
GCCAGTA37050.016.2034361
TCGGGCG39950.015.8042967
GTCGCTA46450.015.3744927
GCCAGCG44350.014.85766534
GGCTGCC47400.014.62946943
GTGCGAG38550.014.58744610
GTCCTAT45100.014.45759743
ATCGGGC44350.014.3926636
CCTATAA46250.014.24729645
GGCGCAT41650.014.08161917
Produced by FastQC (version 0.11.2)