FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt0120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt0120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17913809
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24300.023.56752246
CGCGTAT20000.022.7712251
CGTATGC26950.021.63416344
GTGTGCG30900.020.8792388
CGGGCGC34650.018.8178815
GTATGCC31200.018.687245
CGCTATG49800.018.49679629
GGCGGGC35450.018.3933213
GCCAGTA45550.017.7243351
ATGCCGT35800.016.76788747
CGGCAAT57350.016.5440441
GTCGCTA57300.016.3771627
TCGTATG36200.016.29679143
GCCAGCG53800.016.22376334
TAGCCCG39600.016.2043334
GTGCGAG39550.016.13785710
GCGGGCG40950.015.75432614
GCACACG46100.015.64096611
GTCCTAT56150.015.60624943
AGGTGCC56600.015.36026430
Produced by FastQC (version 0.11.2)