FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt0120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt0120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16572219
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24600.020.47529646
CGTATGC25150.019.8903544
GGCGGGC25550.019.17404713
CGGGCGC26450.018.52145615
GTGTGCG24450.017.6388848
CGCGTAT15500.017.1396371
GCGGGCG28650.016.97879814
GTATGCC31800.016.05635645
CGGCAAT46900.015.6692911
CGCTATG39100.015.26451829
TCGTATG34600.015.15583743
TAGCCCG34200.014.72788834
GCCAGTA38750.014.6911161
ATGCCGT34150.014.64838447
GCACACG44100.014.62924711
ATCGGGC40400.014.3477436
TCGGGCG38550.013.9618017
GGCGCAT38000.013.52758817
CCGACAT53300.013.399411
CGGGCAT29300.013.0706731
Produced by FastQC (version 0.11.2)