FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox60120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox60120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13146743
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC17500.024.44542533
CGTATGC19950.021.96206544
GTGTGCG20850.020.022118
TATGCCG21450.019.94374746
CGGGCGC22250.017.98637415
CGCTATG31500.017.30455829
GGCGGGC23400.017.2501913
GTATGCC26400.016.33497445
GCCAGCG32300.016.23510434
GTCGCTA34650.016.03023727
ATGCCGT27900.015.70405847
GTCCTAT34400.015.54481743
CGCGTAT13400.015.4486371
TCGTATG28050.015.37409343
GCGGGCG26100.015.33321114
GCACACG32200.015.32165811
CCTATAA35550.015.13900745
GGCTGCC37350.014.77888843
TCCTATA37350.014.594151544
AGGTGCC37150.014.57990930
Produced by FastQC (version 0.11.2)