FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox60120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox60120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12577694
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC19550.020.1173515
TATGCCG17300.019.941948
CGTATGC17400.019.6290246
GTGTGCG18350.019.5537768
GGCGGGC20900.018.8179813
GCCAGTA30550.017.9572581
CGCGTAT12000.016.9638671
CGGCAAT38000.016.6158121
CGCTATG32600.016.08565129
TAGCCCG25200.016.0176234
GCGGGCG25050.015.70036714
GTCCTAT34800.015.16787943
GCCAGCG36300.015.01630734
CCTATAA35950.014.97057145
AGGTGCC36700.014.66463230
GCGGAGT37800.014.60307538
CCGACAT42100.014.4239651
ATGCCGT22900.014.31201549
GTCGCTA36400.014.31160127
TCGGGCG30700.014.2726267
Produced by FastQC (version 0.11.2)