FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox1120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox1120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13288645
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20550.022.49600644
TATGCCG21050.021.9616646
CGGGCGC12400.018.08446915
CGCGTAT12150.017.6059931
GTGTGCG17300.017.5497238
GCCAGTA28200.017.1286871
GTATGCC27650.016.96945
TCGTATG28650.016.73796743
ATGCCGT28300.016.57925247
GGCGGGC15650.014.76985313
GCACACG37350.013.85547211
GTCCTAT33100.013.75809843
CGGCAAT35900.013.6470581
CCTATAA34300.013.37734745
GCCAGCG33400.013.11806434
CGCGCGT18750.012.87980465
ACGTCTG40600.012.83113315
TAGCCCG18300.012.81947734
TCGGGCG21850.012.7899057
CGGGCAT21100.012.5908031
Produced by FastQC (version 0.11.2)