FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox1120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox1120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14213499
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG24800.021.980538
CGCGTAT14400.021.5631771
TATGCCG19750.021.48584446
CGTATGC20000.021.21727244
GCCAGTA34850.020.5917531
CGGGCGC28250.019.66173615
GGCGGGC29050.019.1204113
GTGCGAG31200.017.80300510
TAGCCCG32500.017.09064534
CGCTATG37600.017.06630729
CGGCAAT45500.016.9093231
GTATGCC26700.016.15151245
GCGGGCG34400.015.94610614
ACCCGTA33650.015.89135832
TCGTATG28050.015.86613843
AGTAGTC44700.015.2056054
TGCGAGT35400.015.2034411
ATGCCGT27750.015.04308147
TGTGCGA36250.015.0373449
CGTTCAG37950.014.99983957
Produced by FastQC (version 0.11.2)