FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox10120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox10120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12131487
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17250.018.79973646
CGGGCGC16600.018.28897515
TATGCCG18100.017.72626948
GTGTGCG17550.017.4962738
CGCGTAT12000.016.963171
GGCGGGC19000.015.797276513
CGCTATG29200.015.24128529
GTATGCC22150.014.48512347
ATGCCGT23450.014.12346949
CCGACAT40500.013.9709221
GCACACG31550.013.77810711
GCCAGTA27950.013.5783541
GTCGCTA33450.013.40799327
CGGCAAT34600.013.3617991
TCGTATG24400.013.14940545
GCGGGCG22750.013.04162814
GGCTGCC33750.012.98203943
GCGCACC19600.012.84930866
TAGCCCG23400.012.82679634
TCCGTAT26250.012.74849737
Produced by FastQC (version 0.11.2)