FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox10120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox10120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12862082
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19100.027.45497748
CGTATGC20800.025.2110646
GTATGCC25900.020.37992147
GTGTGCG18750.019.1367538
ATGCCGT27850.018.95296349
CGGGCGC18500.018.08884615
TCGTATG29650.018.0350745
GGCGGGC19300.017.69662513
GCACACG34150.016.87090311
CGCGTAT11900.016.8163091
CGCTATG30800.016.46572529
GCCAGTA30150.016.3643361
CACACGT37900.015.56572812
CGGCAAT34350.015.3678941
GCCAGCG33950.015.14109834
ACGTCTG39050.015.10726715
TCCTATA36400.014.69065644
GTCCTAT35650.014.61262643
GTCGCTA34500.014.60000727
CCTATAA35650.014.51585445
Produced by FastQC (version 0.11.2)