FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox0120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox0120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13747595
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG21000.020.536278
TATGCCG18000.018.97461746
CGTATGC19900.018.2031544
CGCGTAT14950.017.7706171
CGGGCGC22500.017.4797115
GGCGGGC24300.016.3270113
CGCTATG34800.015.86180629
CGGCAAT38950.015.8561781
GCCAGCG33900.015.16346634
GTGCGAG28800.015.093719510
GCCAGTA33400.014.8754131
GTCCTAT35300.014.85527843
TAGCCCG26700.014.73009334
ATGCCGT25100.014.15708447
GTATGCC24900.014.13224445
GCGGGCG27900.014.0965414
GCGGAGT38150.013.74571238
AGGTGCC38250.013.70957730
CGGGCAT23450.013.6833721
TCGTATG25600.013.61110343