FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ox0120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ox0120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13373772
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19550.021.17626846
CGTATGC19750.020.61246144
CGCGTAT12000.018.9760251
GTGTGCG18000.017.2503538
CGGGCGC18300.016.77865415
GGCGGGC18550.016.73844713
GTATGCC25100.016.63131545
GCCAGCG33300.016.05840534
ATGCCGT26650.015.66401647
GTCCTAT34800.015.46536943
CCTATAA34200.015.33318645
TCGTATG27600.014.87485843
CGCTATG28550.014.62163329
AGGTGCC36650.014.49645230
TCCTATA37750.014.25681744
CCCTGGT39600.014.2005767
TAGCCCG22800.013.76960634
ACATCCG40100.013.76540455
GCGGAGT39350.013.58955238
GCGGGCG22400.013.55354414
Produced by FastQC (version 0.11.2)