FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko60120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko60120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13231180
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18700.024.72159248
CGTATGC19200.023.53874846
GTGTGCG23450.023.392638
GTATGCC23450.020.0082647
GCCAGTA32200.019.1796191
CGCGTAT13150.018.8907641
GGCGGGC25600.018.59747713
CGCTATG37400.017.89554829
CGGGCGC26900.017.82696515
GTGCGAG30800.017.69801510
TCGTATG26750.017.15304245
ATGCCGT27950.016.41659549
GTCGCTA40000.016.30127727
TGTGCGA35000.016.1656829
GCCAGCG41050.016.13632434
CGGCAAT42650.015.8554961
GCGGGCG30200.015.7647514
GCACACG33950.015.64939311
TAGCCCG32250.015.4045634
CCTATAA43750.015.21922445
Produced by FastQC (version 0.11.2)