FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko60120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko60120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13535411
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG23750.021.9356088
CGCGTAT16300.019.0505391
CGGGCGC27400.019.01254515
GGCGGGC27550.018.90923713
CGCTATG41150.018.77977829
GCCAGTA33300.018.753691
GTCGCTA43000.017.8917127
TATGCCG17350.017.49828346
GGCTGCC45250.016.84945943
GCCAGCG39950.016.40775134
CCGACAT51850.016.3030821
GTGCGAG32050.016.25439510
CGGCAAT44100.016.1951181
AACGTCG48350.016.05479224
CCCTGTG47350.016.02931463
TCGGGCG37250.015.9308937
CGTATGC19200.015.8122544
TAGCCCG33200.015.69101834
GTCCTAT42400.015.622395543
GCGGGCG33400.015.59711714
Produced by FastQC (version 0.11.2)