FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko1120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko1120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17377897
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC27950.020.36653715
TATGCCG25300.020.18151548
CGTATGC25050.020.10748146
GTGTGCG27800.019.2362638
GGCGGGC30600.018.37733313
CGCTATG43200.017.09011829
GCCAGTA42800.016.9286291
CGGCAAT49350.016.6393411
GCGGGCG33850.016.61284414
TAGCCCG34950.016.48482534
CGCGTAT17100.016.343131
GTATGCC32150.016.0961847
GTCGCTA49400.015.1547627
GGCTGCC50050.014.8889343
TCGGGCG42050.014.7686327
TCGTATG34350.014.66353445
GGCGCAT39400.014.53556517
ATGCCGT35100.014.448549
CCCTGTG53350.014.29125763
GACTGTC59650.014.2872634
Produced by FastQC (version 0.11.2)