FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko1120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko1120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18334911
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG26400.019.2106428
GCCAGTA40050.019.1246851
CGTATGC26400.018.68729246
TATGCCG27650.017.84247848
CGGGCGC24650.017.35477615
GGCGGGC26600.016.60136413
ATGCCGT33900.015.06177949
CGCTATG44950.015.04321429
CGCGTAT16450.014.6816871
GTATGCC34600.014.55764147
CCTATAA51800.014.51914245
GCCAGCG51100.014.44801934
GCGGGCG30150.014.18889714
GTCCTAT52800.014.1788243
TCGGGCG33900.014.1463617
TAGCCCG32000.014.01550834
TCCTATA55500.013.79984544
CGGCAAT48350.013.7008861
GTGCGAG37600.013.67150110
GTCGCTA50050.013.64827327
Produced by FastQC (version 0.11.2)