FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko10120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko10120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15346061
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC24100.024.62211646
TATGCCG24350.024.08595848
GTGTGCG24850.020.4090088
CGCGTAT15800.019.4345191
CGGGCGC27300.018.32396115
TCGTATG32850.018.16873745
GTATGCC32600.018.09641347
CGCTATG44400.017.63842229
ATGCCGT33500.017.50725649
GCCAGTA37700.017.4796581
GGCGGGC29100.017.30907413
GCCAGCG44450.016.68709434
GTCGCTA49000.016.33471527
CCGACAT59250.016.2463841
GTCCTAT44800.015.86365343
GGCTGCC49750.015.81086943
CGGCAAT46100.015.6417831
AGGTGCC47750.015.53389830
GCGGGCG32400.015.43963114
GCGGAGT48900.015.30968538
Produced by FastQC (version 0.11.2)