FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko10120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko10120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16342856
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407810.24953410835902856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23300.021.46964548
CGTATGC23950.020.59886646
GTACGTA25900.019.98046535
CGTACGT25450.019.52046234
GTGTGCG24200.018.8188868
CGGGCGC26150.018.47026415
TACGTAA27200.018.26460836
GGCGGGC28150.017.28054613
CCGTACG29200.017.13170433
CGCTATG42500.017.04686429
GTATGCC29550.016.3449447
GCCAGTA37700.016.0152851
GCACACG42050.015.91682511
TCGTATG32450.015.841063545
GCCAGCG44650.015.53066334
GTCCTAT45500.015.01296743
GTCGCTA47800.015.01256227
ACGTCTG44350.014.93565815
CCTATAA47450.014.90494645
GCGGGCG32450.014.88435714
Produced by FastQC (version 0.11.2)