FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko0120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko0120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16660271
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC22600.023.96638133
TCCGCAC24450.022.29407535
TATGCCG24750.022.02384448
CCGCACA25300.021.54538736
CGTATGC26250.020.3710646
GTGTGCG26500.019.789338
GTCCGCA28000.019.34429434
CGCACAT28400.019.07212437
CGCTATG37900.017.65931129
CGGGCGC22200.017.56049315
ACGTCCG33550.016.65841532
GTATGCC33150.016.54721347
GGCGGGC23950.016.27736713
GCCAGTA40350.016.161111
CGCGTAT15900.015.84085751
GCACACG40500.015.67383711
ATGCCGT34950.015.30015349
TCGTATG35450.015.27898945
GTCGCTA44400.015.15193627
CGGCAAT45400.014.9714311
Produced by FastQC (version 0.11.2)