FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_ko0120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_ko0120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15831327
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22400.022.48635946
CGTATGC24600.020.8961144
GGCGGGC22000.018.19082313
GTGTGCG23150.018.1816848
CGGGCGC22100.017.64013315
TCGTATG31800.016.92439543
GTATGCC30450.016.76828845
ATGCCGT32550.016.1104247
CGCGTAT14450.015.5200361
GCGGGCG25550.015.39322514
TCACGTA34100.015.27700530
CGGCAAT43050.015.1473211
GCCAGCG40150.014.43570534
GCCAGTA37100.014.1356861
CGCGCGT25150.014.12906765
TAGCCCG28100.014.119060534
ACGTAGA37400.014.1134832
GCACACG45050.014.0144511
GTCCTAT42200.013.89799443
CTCGTAT39050.013.87061342
Produced by FastQC (version 0.11.2)