FastQCFastQC Report
Fri 17 Mar 2023
HN2YHBGXN_n01_JK183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2YHBGXN_n01_JK183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20870603
Sequences flagged as poor quality0
Sequence length76
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC8450484.048986989019915TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC4029571.9307396149502725TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTTG2526461.21053522028089TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTTG2057170.9856782767608584TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGG2036970.9759995913869859TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC1986900.9520089093736296TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGG1820380.872222043608419TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTTG1750530.8387539162141123TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGG1666860.7986640347669879TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGC1660570.7956502263015592TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTTG1595600.7645203159678712TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTGG1506950.722044303176099TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTTG1471400.7050107752037639TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTGG1462660.7008230667796229TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATTC1327500.6360621204859294TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATTG1189970.5701656056607468TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATTG1186980.5687329685682776TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC1069750.5125630534009966TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTGG1048560.5024100166152363TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTG1018340.48793031998165076TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTGG1011210.4845140315303779TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTGG989940.47432266331739437TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTTG905820.43401716759213904TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTGC834090.3996482516580858TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATTG795620.3812156265921018TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC786500.37684584388865044TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTGG764640.36637178139989535TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATTC753010.3607993501673143TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTTG687390.3293579969874373TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATGG681530.3265502199433337TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGG656530.3145716489360657TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTGC636580.3050127492722659TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGG615580.2949507496261608TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTTG552160.26456351069492334TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTTC486640.23317007179907548TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTGG484320.232058460409601TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGGTTG482340.23110975758582536TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATTG470480.22542712349997743TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATTC465670.22312244643817908TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTTC453860.2174637694943457TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGTTTG452990.21704691522329275TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGGTGG448980.21512555243372702TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTTC421720.202064118607402TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTTG400530.19191108182164165TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTGG393520.18855229051120373TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATTG390780.18723943912880714TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATGG380430.18228031073179823TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGTTGG348750.16710106555138823TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATTG330730.15846691156934947TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGTTTG328620.15745592017633606TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATGC283770.1359663637892973TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTTTTG282970.13558304951706474TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTGC280220.13426540670626527TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGATGG278810.13358981530145536TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGATTG277030.13273694104573788TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTTG253150.1212950100195955TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATGC252490.12097877574500362TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTGC234310.11226795890851836TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTTC233520.1118894360646887TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGGTTG228590.10952726186205546TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATGG226680.10861209903710017TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATTC226330.10844439904299842TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTGTTG215100.10306362494653365TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATTG209870.10055770789181319TruSeq Adapter, Index 3 (97% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)