FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389030
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38340.16048354353021937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC6700.018.54082534
GCAAGCG7150.017.84681125
ATAGTGT13650.016.9436669
GTTCTAA9800.016.9000991
CCGTCTA6450.016.58557757
GTATAGA4950.016.0323311
CGACCCC9550.015.89530315
TTGATCG9800.015.1349179
GATCGTT9250.014.9145611
TAAGTTG9250.014.914565
GACCTAC8200.014.72130745
AGCCGAC9850.014.70851810
GACCCCG10600.014.646852516
AAGCGAC8500.014.60688527
ATGACAT16650.014.5002665
TGATCGT10050.014.41581110
AGACCTA7900.014.41080244
CATTGCA17750.014.3804019
ATTCGCC16600.014.34097516
ACTCGAC8950.014.25720924
Produced by FastQC (version 0.11.2)