FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890372
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTT209280.5379434151798337No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTTTACCCTTTTGTT123120.3164735917285031No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCC117080.300948084142082No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT109490.2814383817280198No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG94600.2431644068999057No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATC85860.22069868896856135No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGG81630.20982569276151486No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAA77200.1984386068992888No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAG76470.1965621796578836No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCA72090.18530361620945246No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCA68840.17694965931278553No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGT67620.17381371241619054No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCT66940.1720658075885802No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAATCAAAAAGCAATGTCGCTA66290.17039501620924682No Hit
GTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGG60290.1549723265538617No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTATCCATCTATATTGTGTGGAGCAAAGA59550.15307019482969753No Hit
CCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGT59200.1521705379331334No Hit
CTTTTCTGGCAGTCAAGCGTTCATAGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAA58420.1501655882779333No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTT58140.14944586276068203No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACG56650.14561589482959472No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTA55550.14278840172610743No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTG55120.1416831089674715No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC53340.1371077110363739No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTT53010.13625946310532772No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT52600.13520557931220972No Hit
GCTCTATCCCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTTAGACTCGCTGGCTCCGT51430.13219815482940964No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCC51250.13173547413974807No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTCCGCTTCATTGAATAAGTAAAGAAAC50270.1292164348293685No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATT50250.12916502586385054No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCT49340.1268259179327838No Hit
GTCTAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGTATAG48230.12397272034653756No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTCTCTTCACAATGTC47690.12258467827755289No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47340.12168502138098876No Hit
CTATTTAGTAGGTTAAGGTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCCAT46220.11880611931198352No Hit
ATTTAGTAGGTTAAGGTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCCATGC45670.11739237276023991No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT44660.11479622000158339No Hit
GACTTAGAGGCGTTCAGCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCCGCAAAAACC44230.11369092724294745No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCCGCAAAAACCAATTATCCGAATGAAC42330.10880707551874218No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAG40900.10513133448420871No Hit
CTTTTCTGGCAGTCAAGCGTACATAGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAA40740.1047200627600651No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG40460.10400033724281378No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG39850.1024323637945163No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCGGCCGAATGA39690.10202109207037272No Hit
GGTTTTACACCCAAACACGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAAGAGGTGCACAATCGACCGATCCT39130.10058164103587008No Hit
GTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA38910.10001614241517263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA2400.050.3068662
GGATCGC2550.048.7054331
CTGGCAG25450.046.3557056
TGTCTGG54700.041.811592
CTGGCCG2800.040.6551135
CCCCTGA2850.039.9449510
GACAAGC15200.039.2640714
GCCTGGC2750.038.8860553
GCATATG15450.038.62972319
AAGCATA15550.038.38129817
ATCGCAG3150.038.328553
GGGCTTT29400.037.7926657
AGCATAT15850.037.6548418
TCAAGGG30300.037.0098551
GGCTTTT30050.036.9713858
ATGACTA15900.036.87507223
CAAGCAT16450.036.7008516
GAGACAA16450.036.6937712
TCGCAGA3300.036.588694
GCTTATA6600.036.07759538
Produced by FastQC (version 0.11.2)