FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484567
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTT26400.5448162999131183No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCC16810.3469076515734666No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTTTACCCTTTTGTT13820.2852030782120945No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT12530.25858137264815806No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAG10480.21627556148066213No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG9620.19852775777137113No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAA9610.19832138796079798No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATC9520.19646405966563962No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGG9490.19584495023392015No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAATCAAAAAGCAATGTCGCTA9430.19460673137048126No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT9030.18635193894755525No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCC8550.176446188040044No Hit
GTCTAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGTATAG8340.17211242201800783No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCT8190.16901687485941058No Hit
CCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGT8170.16860413523826426No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCA7780.16055571262591137No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC7760.16014297300476507No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACG7570.15622194660387523No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT7420.15312639944527795No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTT7410.1529200296347048No Hit
GCTCTATCCCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTTAGACTCGCTGGCTCCGT7400.15271365982413165No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTA7000.14445886740120562No Hit
GTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGG6950.14342701834833985No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTT6900.1423951692954741No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCA6600.13620407497827958No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTCCGCCTAGCAGAGCAAGCCCCACCAA6340.13083845990337764No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATT6270.1293938712293656No Hit
CTATTTAGTAGGTTAAGGTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCCAT6140.12671106369191465No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTATCCATCTATATTGTGTGGAGCAAAGA6090.12567921463904888No Hit
CTTTTCTGGCAGTCAAGCGTTCATAGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAA6040.12464736558618313No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCA6000.12382188634389053No Hit
GTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA5960.12299640710159791No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCGGCCGAATGA5690.11742442221612286No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGT5690.11742442221612286No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG5580.11515435429981818No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCCGCAAAAACCAATTATCCGAATGAAC5510.11370976562580612No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAG5420.11185243733064779No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG5390.11123332789892831No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAG5300.10937599960376997No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGT5270.10875689017205051No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTGTTACACACTCCTT5250.10834415055090421No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTCTCTTCACAATGTC5170.10669319206631901No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCAC5100.10524860339230695No Hit
ATTTAGTAGGTTAAGGTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCCATGC5030.10380401471829488No Hit
CGTCAGAACCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCG4880.10070846755969763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA208.496425E-569.0209664
ATGCGTT150.00235670768.9853430
TTCTAGT200.00734764551.7657243
GCCTGGC200.00734764551.7657243
CTGGCCG200.007350645551.760385
CGGCAAC200.00735364751.75503550
AGATTAT200.00735364751.75503554
ACTGTGT200.00735364751.7550358
TCCGTTC200.00735364751.7550358
CGGTCAG200.00735364751.7550359
GTATGCG200.007362657751.7390128
GGACATA200.007362657751.7390123
CCGCCGA200.007362657751.7390115
ATGACTA1250.049.66944523
TCGCTCT652.1282176E-1047.778815
CATATGA1300.047.75908720
GCGGGTG306.301591E-446.0092246
CCCCTGA306.304799E-446.00447510
CGGGTGA306.308008E-445.999727
AAGCATA1350.045.9902317
Produced by FastQC (version 0.11.2)