FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551705
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG132150.3720748204031585No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCC101810.2866510591392022No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG74610.21006812221172647No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC73680.2074496615006032No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCT72590.2043807129252007No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACG68450.19272433943697465No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCA67500.19004956774281648No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTCCGCTTCATTGAATAAGTAAAGAAAC65520.18447478042236054No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATC61270.17250869652744244No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATT60930.17155140981584902No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTA60150.16935528147748757No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTT54780.15423578253261463No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT53930.151842565753631No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCAC53030.14930857151706012No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCC51900.14612700097558778No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTA51810.1458736015519307No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAA51370.1446347599251627No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTCCGCCTAGCAGAGCAAGCCCCACCAA50600.14246678707831872No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCA50430.141988143722522No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTCTCTTCACAATGTC50240.14145318938369036No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTA49040.13807453040159584No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAG48490.13652597836813588No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAGATGTATTTATTAGATA48440.1363852009105486No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTTTGATTTT48030.13523082575833295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47910.13489295986012353No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG46470.1308385690816101No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAG45170.12717835518434104No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT43460.12236376613485636No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGT43340.1220259002366469No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTG43210.12165987884692No Hit
CTTGTAGACCGTCGCTTGCTACAATTAACGATCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAAT42770.120421037220152No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTT42530.1197453054237331No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG42180.11875986322062221No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAATCAAAAAGCAATGTCGCTA41210.11602878054342913No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTATCCATCTATATTGTGTGGAGCAAAGA40130.11298798745954408No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAG39620.1115520573921539No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT38810.1092714625792401No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTT38660.1088491302064783No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTATA37560.10575202613955834No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGT37300.10501998336010451No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTC36210.10195103478470201No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTCAGAAAGTGATG36040.10147239142890528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGA4200.049.2691767
GCGGGTG4950.041.8041536
GCATATC21750.041.5546261
ATATCTA23650.037.042513
ATCTAAG24000.036.6434365
CCCCTGA2550.036.52281610
CATATCT24000.036.5023082
AGCGGGT5800.036.2717975
CAGGGTG2450.033.784587
TCGGAGT1450.033.3005754
CCCCCTG2950.031.5683449
GCATATG10550.030.7376819
CCGATCG450.004629660430.6589689
TACTGGC10950.030.55397228
ACACGTA3850.030.4654615
ATCGGAG1650.029.2645553
TGACTAC11200.029.25233824
CGCTAGG1551.8189894E-1228.9298546
GCTATTT6050.028.5044363
GCCTAGT1653.6379788E-1227.1937667
Produced by FastQC (version 0.11.2)