FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199691
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG97510.3047481772458653No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGT78690.24592999761539475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57860.18082996139314703No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTA56150.17548569533745603No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCC49860.1558275470975166No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTT42030.13135643410566833No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC38870.12148048045889431No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT35950.1123545992409892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGA3300.061.679467
GCGGGTG3950.052.4046946
AGCGGGT4200.051.7488335
ATATCTA17300.047.264153
CATATCT17650.046.5223732
GCATATC17750.046.4590031
ATCTAAG17650.046.324735
TGACTAC16600.045.7111424
TACTGGC17000.043.82539728
CAGGGTG2450.043.651397
ATGACTA17600.042.72263323
GCATATG17750.042.563919
TGGGGCA4250.041.3971214
GGGCAAA4400.039.98647716
CTGTCCG350.001348328239.4276775
TCGGAGT801.6425474E-938.8134424
CATATGA20000.037.9467720
GACAAGC20450.037.4497214
ATATGAC20650.036.74772321
GAGACAA21050.036.37658312