FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6994142
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG16450.015.93826816
CGACTGT19950.013.31500217
CGAATAG76350.012.88572669
ACGGCCC13900.012.41058210
TCGGGTG13550.012.22382841
CCCCCCA13050.012.1604619
TAGCCTA10800.012.13938710
GAATAGA80650.011.98468969
GGGATCG14800.011.89078837
CGGTTCG9650.011.80018437
TACGGCC14400.011.7391429
ACGTGTG18750.011.593719542
ACCCCCC13150.011.5430448
CGGCCCA15400.011.19855511
AATAGAG70600.011.1481669
AGCCTAT12100.011.1171111
TATACTG25200.011.0883135
GTATTAA25300.011.0463821
GACGTGT20650.011.02882341
GCACCCC14100.011.0096726
Produced by FastQC (version 0.11.2)