FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5194256
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55300.10646375534821542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCCC8450.013.06533710
GTATATA21050.012.9499851
GTATAAA23700.012.0843711
GTATAGA15950.011.8986111
GTATTAG14450.011.7009881
CGGCCCA9800.011.61477111
GTATTAA17100.011.5019911
TACGGCC9950.011.4417689
TATACTG21850.011.209665
CTAATAC14200.011.1752993
TAGAGCG10250.011.1064595
GGAGCGC6850.011.07772822
TATTATA31550.011.0437342
TAATACT20350.011.018914
CAGCGCG2550.001532017610.823119
TAGGACA13550.010.6930034
TTATACT23600.010.6708884
GTATAGG12350.010.6172221
TAGGCCG2600.00180167910.6145655
CGAATAG36750.010.52127469
Produced by FastQC (version 0.11.2)