FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5916787
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66270.11200335587541008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG13300.015.5657431
TATACCG9550.015.5324465
TATAAGG16850.014.536962
GAATAGA112500.014.20766669
AATAGAG94350.013.64775669
TATAAAG18600.013.54022
CGAATAG125300.013.11445469
GTATAGA13900.012.9079951
TAGAGAG50100.012.81596168
GTATTAG10800.012.7792831
TATACGG13400.012.6143855
GCCTAGG3852.2810127E-812.5469321
TCTATAC15150.012.5244273
TATATAC9700.012.4481313
TAGACCG6100.012.4413535
TATACAG10100.012.2957745
TAATACT12350.012.2912844
TAGCCTA6300.012.04659410
ATAAGAC13850.011.9563683
CCGAGAT13900.011.91084312
Produced by FastQC (version 0.11.2)