FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351163
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3830.1090661601592423No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATA200.007347652751.762722
GGGCGCG200.0073642351.7332311
GCTTAAG400.002590763134.508482
GTAACTA400.002592574834.5035634
GTATACA802.7461174E-630.1992231
TAAGTTA500.007734576727.59498462
TATATAC658.632662E-426.5449853
CAGTATG658.6398504E-426.5412034
TAGACTT1057.6511424E-726.288435
ATAATAA809.5181196E-525.8850481
GTATAAC809.5181196E-525.8850481
CGTAGAC700.001330095724.6489123
GGACCAG700.001331200924.6454015
CCCCTAA851.4367208E-424.36241
AGGTGTA1605.6579665E-821.57394869
TAAGGTT1154.5872897E-521.0021694
CTCCTGA850.004105329520.3021
TTAGACC850.004108719520.2991073
CTGGTCG850.00411550720.2933229
CCCGAGC850.004118904520.29043229
Produced by FastQC (version 0.11.2)