FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5333147
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55610.10427239301673104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA14800.016.0872021
GTATATA11150.015.4734911
GTATAGG8450.014.2923771
TAGCCTA6800.014.20683910
TAGAGCG11150.013.923925
ACGGCCC11350.013.37534910
TCTATAC14750.013.3327253
TAATACT13350.013.1802914
CTAATAC12000.012.9380063
GTATAAG12850.012.8893581
AGGCCCG5451.8189894E-1212.660695
GTCGCGA6850.012.59338866
GTATACA18100.012.3916151
CGAATAG46300.012.37608368
CGGCCCA12550.012.36821811
AGAGCGT14250.012.3473896
CTATACT11250.012.2671474
TATATAC11550.012.2472323
TATACTG16400.012.2013535
GTATAAC19250.012.1890911
Produced by FastQC (version 0.11.2)