FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5541229
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72430.13071107510626254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTA11600.016.05902310
GCGACTG11500.014.39920516
GTATATA22750.014.255961
TAGGACA12600.014.2362814
TCTATAC17650.013.4866293
AGCCTAT14400.013.17326411
TACGGCC10300.013.0614439
CCTATGA16200.012.98885213
GACCTAT14000.012.8119587
TCCTAAC18000.012.6494162
TATACTG24250.012.517545
CTCCTAA18200.012.511881
CGGACAC11850.012.22893959
GACACGG9900.012.1947567
CGTAAGG20550.012.0870332
CGGCCAG6000.012.075861
AGGACAG15150.012.0673335
CGGCCCA11150.012.06376711
ACGGCCC10950.011.97161810
CGCTAGG4359.789801E-911.89482146
Produced by FastQC (version 0.11.2)