FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6373976
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89560.14050884408726988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTGT54150.017.78635869
AGAGGTG92300.016.344169
GTGTAGA22250.015.20468369
TAGAGCG12850.014.7665265
GAGAGGT102050.014.6810869
AATAGAG230250.014.30309569
TAGAGAG136850.013.8739269
GAATAGA285150.013.76475269
ATAGAGA183950.013.75578869
GTATAAG14250.013.56203651
AGAGAGG118050.013.27611969
GTATTAG12650.013.0949081
TCCGGGC6350.013.03547423
CGAATAG334000.012.82645569
TAGACAG14150.012.6784355
GAGCGTA14500.012.6096447
AGAGCGT15900.012.5847986
CTCCGGG6700.012.35519522
TATACCG10400.012.274035
CTTACAC16350.012.2396763
Produced by FastQC (version 0.11.2)