FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422543
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9550.2260125005029074No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAG450.0046205830.66508717
TAGCCTA605.4105994E-428.75532510
CAAGGGG500.00772248427.6051164
ACAAGGG809.543458E-525.8767323
CTCCTAA951.0472109E-525.4257641
AGACTGC902.1205984E-423.0042616
GACTGCT1052.273104E-523.0042617
CTTATAG800.002895305621.5639443
TTACACT1455.2672294E-721.4177634
TACACCG1154.5872308E-521.0038937
TCCTAAC1004.3266E-420.7013852
ATGTACA1258.7119435E-519.323584
TACACTA1301.17710864E-418.5803665
CTAGCAC1351.5731477E-417.8900853
GTGTTAA1753.1924228E-617.7461451
ATATGGC1200.001474025417.2511543
AATACCG2251.1003249E-716.8697935
GTCACCG1250.001935475616.5630688
GAGAGGT5250.016.43356168
AATACAG1704.9411174E-516.2383025
Produced by FastQC (version 0.11.2)