FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524262
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA67650.1495271494002779No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT60250.13317089063365473No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC49330.1090343574266919No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47270.10448112863490222No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC46090.10187296845319745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGA5550.028.5909027
GCGGGTG6350.026.0768136
ACGCGTG4300.024.08716469
CCACGCA10000.022.0786139
CCCACGC9500.021.7876228
CCCCACG9800.020.7677277
TATACGG17500.018.332735
AGCGGGT9250.018.2741245
ATCACTG38250.018.218065
CCATCAC38000.017.9759833
CAAGGGG14150.017.31465352
TGCGTGG6800.017.25044310
GGGGCTT15100.017.14037156
CATCACT41400.017.0828274
AGGGGGC14600.016.54554454
TACGCGT8350.016.5388969
GATAGGC15550.016.4167946
TATCCCC12800.016.1713964
ACTGTAC46250.015.6635348
ATCCCCA13900.015.3871815
Produced by FastQC (version 0.11.2)