FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524998
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA45390.10030943660085595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG16500.020.069315
ACGCGTG3001.8189894E-1218.4127469
AGGGGGC7200.018.21328554
TAGGCCG1200.001479371917.2470635
GGGGGCT7900.017.0362855
TCATATA26350.015.5786812
CCATCAC29150.015.3836563
CAAGGGG8600.015.24816452
ATATACG21750.014.7498644
CGATAGG9100.014.0250845
ATAGGCC9400.013.9438167
AAGGGGG9850.013.66361453
GATAGGC9350.013.6500826
ATACGGT26800.013.6431996
CATATAC28100.013.6260933
ATCACTG33450.013.6120315
TATACCG7550.013.2493985
ACTGTAC34250.013.1929378
CATCACT35100.013.169284
CACTGTA32750.013.1651137
Produced by FastQC (version 0.11.2)