FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4880810
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG83110.1702791135077989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76940.15763776914077787No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT55450.11360819208287148No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA53310.10922367393936662No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG5250.027.61663869
CGCGTGT5250.017.75355369
TATACGG11900.017.6837485
TACGCGT9100.017.44953568
CTCGACT50750.017.12520425
GTATATA21700.017.0139241
ACTCGAC52450.016.6360724
TCGACTC53300.016.24118626
CATTCCC55150.016.07323812
GTATTAG11400.016.0417391
GTCGTGA14350.015.62426131
AACTCGA56500.015.50493223
CGTGAGC13400.015.44408712
AGGCACT55350.015.33644238
CTCTTCG57600.015.33000130
CCATCAC33850.015.2867483
GGCACTT55400.015.19787339
CGAAGGC58450.014.99457335
TTACGCG11550.014.94406969
GCATTCC60800.014.57899211
Produced by FastQC (version 0.11.2)