FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966812
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA75170.18949725875589768No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT71980.18145553658706284No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG57420.1447509990390268No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC53250.13423877915061264No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC53240.13421356999020875No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTT44570.11235722792005268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCAC33600.021.046653
CCGTGCG1507.345261E-720.6988899
CACTGTA36650.019.7657667
CATCACT36150.019.3719164
ATCACTG37300.019.0511555
TATACGG9800.017.9517575
GTATATA13550.017.8241671
ACTGTAC40550.017.7805718
TCATATA12650.017.7252032
ACCGCGC2359.507858E-917.6158528
TCACTGT41200.017.5826766
TCCATCA41550.017.2687422
CCGCGCG1452.7256453E-416.6542769
ACGCGTG3552.7284841E-1115.55807469
CCGCGCA2907.548806E-915.4646859
TAGAGCG6250.015.45399955
GAGCCCC5450.015.1906965
GACCGCG2756.595474E-815.0527887
CTGTACT47050.014.9577519
CTAATAC9700.014.5807713
Produced by FastQC (version 0.11.2)