FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4985733
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA75350.1511312378741501No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT72480.14537481248995884No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG58390.11711417358290147No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC55190.11069585956568473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54750.10981334138831743No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTT52530.10536063603887333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGA8000.037.9397
GCGGGTG9600.031.9757446
AGCGGGT12450.026.8719985
ACGCGTG5250.021.04231869
TATACGG10950.018.8988195
CACTGTA37200.018.7284417
GACGCGC7950.018.66732838
CATCACT39450.018.011074
TACGCGT10000.017.95172769
CCATCAC39050.017.8417153
CCCCCCT9100.017.814238
ATCACTG41750.017.6788835
ACTGTAC40350.017.6089848
GAGCCCC10600.017.5705555
CGCGCAT8550.017.3573440
GCCCCCC9150.017.3392357
TCCATCA40100.017.2888722
GGGGCTC12100.017.10402310
TCACTGT44350.016.020486
GTCCTAG4750.015.97721
Produced by FastQC (version 0.11.2)