FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4526809
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49050.1083544722121035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGA4900.029.5676467
ACGCGTG8850.027.30546469
CGCGTGT7200.023.97354569
GCGGGTG6450.022.999096
AGCGGGT6900.021.999135
TGCGTGG13600.021.05478110
TACGCGT15400.020.1751469
TTACGCG21100.019.63331269
CGCTCGA2002.8867362E-818.97466910
CCACGCA14650.018.8380159
CCCCACG14150.018.771457
CGTGGGG11950.018.76142312
GAGCCCC11300.018.6231585
GCGTGGG12650.018.2673611
CCCACGC14350.018.0292518
TATACGG9800.017.6013455
ACCCCCC5000.017.2479848
CTTAAGG14300.017.1296393
TTGCGTG18350.016.355589
GTGGGGC11400.016.3383913
Produced by FastQC (version 0.11.2)