FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n02_a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n02_a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107412
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2680.2495065728224035No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA150.002350411868.9948711
GAGACAC200.007329603751.77026742
TCGTATG200.007329603751.77026742
TGCTTAG200.00735666851.72206523
CGTGTAG306.2592543E-446.0394769
CTGCTCA306.27366E-446.0180179
ACGCGTG1102.7284841E-1134.52960269
GTAGTAA400.002580940334.513511
TGCTTCG400.00259276834.48137721
CGGTATG400.00259276834.48137726
GTAAGGT450.00459541730.6786794
ACCGAAT450.00461642530.65011612
TACGCGT1900.029.0775669
ACGCTTA605.3775817E-428.7612616
CCGAATT605.4070086E-428.73448213
TCGGTAT605.4070086E-428.73448225
CGCGTGT500.00767202427.62368267
CACTGTA500.0076895127.610819
TTACGCG2200.025.11243868
TCTGACT700.001330172824.62955548
Produced by FastQC (version 0.11.2)