FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389030
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACAT16450.017.2013535
GTTCTAA9250.016.7913421
TAGGGGT1855.440472E-616.7874774
TAAGTTG8700.016.6588365
GCATATG5850.016.51299519
CCACGCA6750.015.8436189
TTGATCG9800.015.8410119
GATCGTT8300.015.79435611
GCAAGCG7550.015.53593825
AAGCGAC7350.015.48898827
AATTCGC18200.015.35359515
CAATTCG18550.014.87793114
CATTGCA19100.014.8107459
TGACTAC6800.014.71369924
ATTCGCC17850.014.6883116
GGTCTAC7050.014.68100734
TGATCGT8700.014.67164910
CTTAGGG1657.2634674E-414.6395833
CGTATGC4950.014.63682641
CGTGCGA1901.2831151E-414.52555716
Produced by FastQC (version 0.11.2)