FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544528
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCC142620.40236669028993427No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTT119190.3362648002780624No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC82940.2339944838917904No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCC79320.2237815585036992No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCT79210.22347122099190642No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG75350.2125811955780854No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCA74540.21029598299124735No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATC72100.20341213272966102No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT71230.20095764513639053No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAG68550.1933966948490744No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATT67360.19003940722149748No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACG65790.1856100445531817No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAA65270.1841429944974338No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT64080.18078570686985687No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCAC62660.1767795317176222No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTA60790.1715037940171442No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGG58830.16597414380701747No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG58010.1636607187191073No Hit
GTCTAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGTATAG56770.16016236858617No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT56320.15889280603792663No Hit
GGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTTTACCCTTTTGTT55120.1555073059092776No Hit
ATCCAACGCTTACCGAATTCTGCTTCGGTATGATAGGAAGAGCCGACATCGAAGAATCAAAAAGCAATGTCGCTA55000.15516875589641274No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAG54500.15375813084280898No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTCCGCCTAGCAGAGCAAGCCCCACCAA53710.15152934325811504No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCA53160.14997765569915092No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTT52640.14851060564340302No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTT51510.14532259302225853No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTCTCTTCACAATGTC50570.14267061792148347No Hit
TTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGTATCCATCTATATTGTGTGGAGCAAAGA50410.1422192179043303No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG50240.141739605386105No Hit
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCGGCCGAATGA49630.14001864282070842No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTTTGATTTT44950.1268151923189773No Hit
ACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAG44020.12419142971927433No Hit
CCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGTTCAGCGACGCCACAAGGACGCCTTATTCGT43710.12331684218604001No Hit
GTATAGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCCGGTGAGTTGTTACACACTCCTT43490.12269616716245435No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTCCGCTTCATTGAATAAGTAAAGAAAC42970.12122911710670645No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGT42740.12058022958204873No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG42510.11993134205739099No Hit
ATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAGGGAATCCGGTTAA42370.11953636704238194No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAG41810.11795646698234574No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTC40700.11482487936334543No Hit
GTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGG40480.11420420433975977No Hit
GCTCTATCCCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTTAGACTCGCTGGCTCCGT40400.11397850433118317No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGT38910.10977484167144398No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG38690.10915416664785833No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTGGCATGAAAACTATTCCTTCCTGTGG38160.10765890409103836No Hit
GTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA37770.10655861654922742No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAA37410.10554296651063273No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTG37400.10551475400956065No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAG37190.10492229148704707No Hit
TACCTATACTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTA37020.1044426789688218No Hit
GCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCCGCAAAAACCAATTATCCGAATGAAC36590.10322954142272257No Hit
GACTTAGAGGCGTTCAGCCATAATCCAGCGGATGGTAGCTTCGCGGCAATGCCTGATCAGACAGCCGCAAAAACC36590.10322954142272257No Hit
GTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACC36340.10252422889592071No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCTC150.002356275568.9976265
GCGGGTG4650.061.5950246
CGGGTGA4900.057.7326977
CGTCCCG200.007361296651.74821542
AGCGGGT6600.044.4421965
TACCGGG651.1397788E-842.460079
ACACGTA4700.041.83897815
CACGTAA4800.040.96733516
TGCCTAG1300.039.80631666
CCCCTGA2450.038.01909310
CGCTAGG1550.037.83740246
ATGACTA7800.037.1536123
GCATATG7900.037.11897319
AACACGT5400.035.77654314
TACTGGC8550.034.70153428
TAGACTA400.002594089634.5080575
CCGGGGA709.744326E-734.49978324
ACCGCGC400.002597501734.498818
GTGATCG1600.034.4988152
GCTTATA5000.034.49880638
Produced by FastQC (version 0.11.2)