FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199691
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG101310.31662432403628976No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGT74290.232178669752798No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTA60050.18767437230657585No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCC50310.15723393290164583No Hit
GTGTCTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTT45810.14317007486035369No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC38470.12023035974411278No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAGTGCACCGTTGCTAGCCTGCTATGGT34750.10860423709664466No Hit
GTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTATA32000.1000096571825217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTG3200.066.863916
CGGGTGA3800.056.2870867
AGCGGGT4350.049.9805835
GCATATC17800.048.290131
TCGGAGT950.047.2247624
ATATCTA18500.045.3298843
CATATCT18900.044.7378042
ATCTAAG18950.044.435565
TACTGGC14850.044.3725528
TGACTAC15000.043.92882524
GGGCAAA4450.043.4138916
AAAGGGG2650.042.96044568
TACCGGG651.1399607E-842.4597439
CAGGGTG2100.041.069697
CGTATGC3900.040.69185641
ATGACTA16250.040.54905323
CGGAGTG1200.040.2621355
TGGGGCA4800.040.24829514
GCATATG16700.040.0761519
CATCCAG2850.039.9712871
Produced by FastQC (version 0.11.2)