FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6994142
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG18200.014.785116
GACGTAG66750.014.26477969
CGTAGAG37950.014.18143169
ACGTAGA56150.013.88564169
GGAGCGC10900.013.60981822
ACGGCCC14600.013.46871610
TAGGACA14800.013.0573394
TACGGCC14850.013.0096549
GTATAGA23050.012.578171
CGGCCCA15450.012.28104311
CGACTGT23100.012.24624417
CTAGGAC9050.012.2019643
CGTGTGT19250.012.18668143
TTAGGAC12850.012.0847523
ACGTGTG19550.011.82320842
GAGCGCT13350.011.6289823
AGACGTA86500.011.60604269
GTATATA24500.011.5519921
GTATAGG13750.011.5468691
GACGTGT21850.011.52616641
Produced by FastQC (version 0.11.2)