FastQCFastQC Report
Thu 10 Aug 2017
HN2WTAFXX_n01_b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN2WTAFXX_n01_b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5194256
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG12050.014.8873716
GTATATA19750.014.3307891
GTATTAG13450.013.34456351
ACGGCCC9100.012.51060910
ACGTGTG13300.012.19124142
GTATAGA17600.011.9630111
GGAGCGC8450.011.83987622
TAATACT19950.011.7623244
TACGGCC10050.011.6712889
CTAGGAC7250.011.4235313
CGTGTGT14250.011.13639643
TTATACT20950.011.0361584
CGACTGT16350.010.97203717
CTAATAC15150.010.9334133
CGGCCCA10800.010.86078111
GTATAAT35050.010.8324821
TATACCG9900.010.8057185
TATACTG21300.010.69280055
TCGCCAG14200.010.69002267
TAGCCTA8750.010.645390510
Produced by FastQC (version 0.11.2)